Table 4 Specific amplification markers of chromosome 2St and chromosome 3St
Specific primers | Primers (5’-3’) | Amplified chromosomes | Annealing temperatures |
|---|---|---|---|
PTH-005 | F: TCCTCAACTGGAAACAAAGGA | 2St | 56 |
R: TTGGGAGTGAGTGTAGTTCAC | |||
PTH-013 | F: AGCCCTCCGGAAAGAATGAA | 2St | 62 |
R: CCGCTCAAACAATCGCTACC | |||
PTH-113 | F: AACAGGGTCAACGGGTTTGA | 3St | 60 |
R: TTGGTGCAGAAACAATGCGG | |||
PTH-135 | F: TGCCTCTAACACATGCATGT | 3St | 60 |
R: TCCAGTAGGTCTTGGCTCCA |