Fig. 7

SOS3 and ABI5 expression is activated in the presence of SmAP2-17 as measured by the luciferase reporter assays. a Schematic diagrams of the putative AP2/ERF binding elements in the 2000-bp promoter region of the five candidate downstream genes. b Left panel: Schematic diagrams of effector and reporter plasmids used in transient transactivation assays: top, schematic diagrams of control (EV, empty vector) and effector (RV, recombination vector); bottom, schematic diagrams of reporter constructs. The promoter sequences from five candidate downstream genes were cloned upstream of the LUC reporter gene. The REN located downstream of the 35S promoter was used as an internal control. Right panel: The results showed that five candidate downstream genes, SOS3 and ABI5, were regulated by SmAP2-17. Relative activity was calculated as LUC/REN, but was normalized to the reporter only. Data represent the mean ± SD of three biological replicates. *P < 0.05 and **P < 0.01 by Student’s t-test