Fig. 7

a. Validation of the relative expression levels of five selected genes responsive to combined DTHT stress from RNA-Seq analysis by quantitative real-time PCR (qPCR). The genes selected were differentially expressed, and the time point at which these genes showed high expression from the RNA-Seq data were selected with its control for qPCR validation. b. Validation of relative expression of DT-responsive gene UDP-glucosyl transferase 85A3. UDP-glucosyl transferase 85A3 was up-regulated and down-regulated at different time points during DT stress from the RNA-Seq data. The expression pattern of the qPCR analysis is like results from the RNA-Seq analysis. The different alphabets in the Figure show that the samples collected from the different time point of DT are significantly different from the control at p-value< 0.05. qPCR results from two technical replicates and three biological replicates were analyzed using ANOVA from Minitab 18 software. The x-axis shows the treatment imposed on switchgrass. The y-axis shows the relative expression of the genes