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Fig. 3 | BMC Plant Biology

Fig. 3

From: ROS1-mediated decrease in DNA methylation and increase in expression of defense genes and stress response genes in Arabidopsis thaliana due to abiotic stresses

Fig. 3

Analyses of the expression levels of related genes. A Analyses of the expression levels of ACD6 and GSTF14 in the mutants ago4, met1, drm1/2, and cmt3 by northern blotting; wild-type Col-0 ecotypes served as background controls for the mutant genotypes. B Analyses of the expression levels of ACD6, ACO3, and GSTF14 by RT-qPCR in DNA methylation mutant plants ago4, met1, and cmt3 with wild-type plants as a background control for the mutant genotypes. C The cold stress-treated ros1 mutants exhibited deformed leaves and increased anthocyanin accumulation on the 7th day compared with the cold stress-treated Col-0 plants. D The related genes were analyzed in untreated Col-0 plants, Col-0 plants treated with cold stress, and ros1 and ros1 dml2 dml3 (rdd) mutant plants treated with cold stress by RT-qPCR. Means identified by different letters are significantly different from each other. Error bars represent SEs from three biological replicates. One-way ANOVA followed by Tukey’s Multiple comparison Test was used for statistical analysis (P < 0.05)

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