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Table 2 Coding sequence variants identified in chickpea mutant PM405

From: Defining the mutation sites in chickpea nodulation mutants PM233 and PM405

Chromosome

GenBank Sequence

Position

Ref

Alt

GeneID

Top tBLASTn Hit

Stop

Frameshift

Syn/NonSyn

Ca2

CM003629.3

37,700,934

G

T

Ca_06416

Heat Shock Protein 90–2

No

No

Syn

Ca2

CM003629.3

38,602,731

CAA

CA

Ca_06500

Predicted NLP1

No

Yes

NonSyn

Ca3

CM003630.3

42,275,344

C

T

Ca_09456

Component of oligomeric protein

No

No

NonSyn

Ca6

CM003633.3

19,663,461

A

T

Ca_18764

Glutathione S-transferase-like protein

No

No

Syn

Ca6

CM003633.3

20,028,458

T

G

Ca_05252

Predicted: serine/threonine-protein kinase GRIK2-like

No

No

NonSyn

Ca6

CM003633.3

57,850,387

TAA

–

Ca_20904

Predicted: phosphatidylinositol/phosphatidylcholine transfer protein SFH3

No

No

NonSyn

Ca6

CM003633.3

58,492,000

A

C

Ca_20961

Predicted: 4-coumarate--CoA ligase 1-like

No

No

NonSyn

Ca7

CM003634.3

36,911,781

A

G

Ca_23613

Predicted: ATP-dependent helicase BRM isoform X1

No

No

Syn

Ca7

CM003634.3

6,585,411

G

A

Ca_21616

Uncharacterized

No

No

Syn

Ca7

CM003634.3

7,929,900

G

A

Ca_21755

Uncharacterized

No

No

Syn

  1. Legend. Top tBLASTn hits were taken from the NCBI nucleotide collection database. The position coordinates are those of the ICC 4958 v3.0 genome reference assembly. Ca_06500, the primary candidate gene, is indicated in bold. The Ref (reference) allele is the allele in the ICC 4958 v3.0 genome reference assembly. The Alt (alternate) allele is the allele seen in the PM405 Illumina reads