Table 2 The primers used in this study
From: Biosystematics studies on Elymus breviaristatus and Elymus sinosubmuticus (Poaceae: Triticeae)
Gene | Name of primer | Sequence of primer (5’–3’) | Profiles |
|---|---|---|---|
Acc1 | AccF1 | CCCAATATTTATCATGAGACTTGCA | 1 cycle: 5 min 95℃; 35 cycles: 30 s 95℃, 30 s 56℃, 2min30s 68℃; 1 cycle: 10 min 68℃ |
AccF2 | CAACATTTGAATGAAThCTCCACG | ||
DMC1 | TDMC1e10F | TGCCAATTGCTGAGAGATTTG | 1 cycle: 4 min 95℃; 35 cycles: 1 min 95℃, 1 min 52℃, 1 min 72℃; 1 cycle: 10 min 72℃ |
TDMC1e15R | AGCCACCTGTTGTAATCTGG |