Fig. 5
Nuclear activity is required for function of MeGRXC3 in transgenic Arabidopsis. (a) Schematic diagram represents DNA constructs of NLS:MeGRXC3:GFP and MeGRXC3:3 × GFP. NLS, SV40 T large antigen nuclear localization sequence. (b) Subcellular localization of NLS:MeGRXC3:GFP and MeGRXC3:3 × GFP fusion proteins in transgenic Arabidopsis. (c) Seed germination assay of NLS:MeGRXC3:GFP and MeGRXC3:3 × GFP transgenic Arabidopsis. Seeds of three independent homozygote lines sown on 1/2 MS medium supplemented with 0 mM, or 100 mM D-mannitol respectively, incubated at 22 °C for 14 days. (d) Effects of mannitol stress on germination rates. Error bars indicate mean ± SD (n = 3). ** p ≤ 0.01 (Student’s t-test). (e) Post-germinated seedling development assay of NLS:MeGRXC3:GFP and MeGRXC3:3 × GFP transgenic Arabidopsis. Seedlings grown on 1/2 MS medium were transferred to 1/2 MS supplemented with 0 mM or 100 mM D-mannitol at 7 days after sowing respectively, then incubated at 22 °C for 14 days. (f) Effects of mannitol stress on seedling biomass. Error bars indicate mean ± SD (n = 5)