Fig. 2

Overexpression of MeGRXC3 confers mannitol sensitivity in transgenic Arabidopsis. (a) Seed germination assay of MeGRXC3-OE transgenic Arabidopsis. Seeds of three independent homozygote lines sown on 1/2 MS medium supplemented with 0 mM or 100 mM D-mannitol respectively, incubated at 22 °C for 14 days. (b) Effects of mannitol stress on germination rates. Error bars indicate mean ± SD (n = 3). ** p ≤ 0.01 (Student’s t-test). (c) Post-germinated seedling development assay of MeGRXC3-OE transgenic Arabidopsis. Seedlings grown on 1/2 MS medium were transferred to 1/2 MS supplemented with 0 mM or 100 mM D-mannitol at 7 days after sowing respectively, then incubated at 22 °C for 14 days. (d) Effects of mannitol stress on seedling biomass. Error bars indicate mean ± SD (n = 5). ** p ≤ 0.01 (Student’s t-test)