Fig. 7From: Genome-wide analysis of the NAAT, DMAS, TOM, and ENA gene families in maize suggests their roles in mediating iron homeostasisSubcellular localization of ZmNAAT, ZmDMAS, ZmTOM, and ZmENA proteins in maize mesophyll protoplasts. GFP was fused to the C terminus of each gene, and the fusion proteins were co-expressed with an mCherry-fused endoplasmic reticulum (ER) marker in maize mesophyll protoplasts. The GFP signal is shown in green, the ER marker is shown in red, and chlorophyll autofluorescence (Chl) is shown in blue. The images were obtained by confocal microscopy. Z-stacked (Z) and single optical (S) slides of the merged channels are shown. The cytoplasmic localization of GFP was used as a control. The scale bar represents 10 μmBack to article page