Fig. 6
Cross sections via the single locule of Petunia anther at the tetrad stage (Te) show: immunogold localization of CRT protein (a, b, d, e), double labeling (c) of CRT protein (20 nm gold particles) and callose (10 nm gold particles), localization of exchangeable Ca2+ by potassium antimonite precipitation (f-i), and the control of immunolabeling (j, k) and potassium antimonite precipitation (l). Arrows in a show immunolabeling at cell peripheries of meiocyte close to callosic cell wall (caw), and arrows in e show immunolabeling associated with perichromatin areas. ch chromatin, cw cell wall, cy cytoplasm, er endoplasmic reticulum, no nucleolus, nu nucleus, Ta tapetum cell, v vesicle. Scale bars correspond to 500 nm