Fig. 5
Cross sections via the single locule of Petunia anther at the dyad/tetrad stage (Dy/Te) show: methylene blue staining (a), immunogold localization of CRT protein (b-d) and localization of exchangeable Ca2+ by potassium antimonite precipitation (e-g). Arrows in d show immunolabeling at cell peripheries of meiocyte close to callosic cell wall (caw). ch chromatin, cy cytoplasm, er endoplasmic reticulum, no nucleolus, nu nucleus, Ta tapetum cell, v vesicle. Scale bars correspond to 20 μm (a) and 500 nm (b-g)