Fig. 4
Cross sections via the single locule of Petunia anther at the microsporocyte stage (Msc) show: methylene blue staining (a), immunogold localization of CRT protein (b-i) and localization of exchangeable Ca2+ by potassium antimonite precipitation (j-l). Arrows in d, f, and g show immunolabeling associated with plasmodesmata, and arrows in j shows Ca2+ ppts associated with plasmodesmata. ch chromatin, cw cell wall, cy cytoplasm, er endoplasmic reticulum, m mitochondrium, no nucleolus, nu nucleus, Ta tapetum cell, va vacuole. Scale bars correspond to 15 μm (a) and 500 nm (b-l)