Fig. 3

Cross sections via the single locule of Petunia anther show: methylene blue staining (a, e, i, m, q), localization of PhCRT1 mRNA transcripts using FISH (green, b, f, j, n, r) and localization of CRT protein using immunofluorescence method (red, c, g, k, o, s) and immunogold-silver method (black traces, d, h, l, p, t) during subsequent stages of pollen development (Msc microsporocyte, Dy/Te dyad/tetrad, MspE early microspore, MspL late microspore, Pg pollen grain stages). Nuclei are stained by Hoechst 33342 (blue, c’, g’, k’, o’, s’). Aw anther wall, Ta tapetum. Scale bars correspond to 15 μm (a-d), 20 μm (e-p), and 25 μm (q-t)