Fig. 2From: Calreticulin expression and localization in relation to exchangeable Ca2+ during pollen development in PetuniaNorthern blot analysis of PhCRT1 mRNA transcript levels (a) and western blot analysis of CRT levels (b) in whole Petunia anthers during subsequent stages of pollen development (Msc microsporocyte, Dy/Te dyad/tetrad, Msp microspore, Pg pollen grain stages) and in dry pollen (Pd). a Graphs show the relative PhCRT mRNA levels (mean of three replicates and standard deviation) normalized to the Ph18S rRNA levels. Statistical analysis was carried out by one-way ANOVA (ns not significant, *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). Cropped representative northern blots are shown under the graphs. Blots were hybridized with PhCRT1 antisense probe, and then re-hybridized with Ph18S rRNA probe. b Graphs show the relative CRT levels (mean of three replicates and standard deviation) normalized to the Petunia glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels. Statistical analysis was carried out by one-way ANOVA ANOVA (ns not significant, *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). Cropped representative western blots are shown under the graphs. Original northern and western blots are shown in the Fig. S1 (Supplementary information)Back to article page