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Fig. 2 | BMC Plant Biology

Fig. 2

From: Calreticulin expression and localization in relation to exchangeable Ca2+ during pollen development in Petunia

Fig. 2

Northern blot analysis of PhCRT1 mRNA transcript levels (a) and western blot analysis of CRT levels (b) in whole Petunia anthers during subsequent stages of pollen development (Msc microsporocyte, Dy/Te dyad/tetrad, Msp microspore, Pg pollen grain stages) and in dry pollen (Pd). a Graphs show the relative PhCRT mRNA levels (mean of three replicates and standard deviation) normalized to the Ph18S rRNA levels. Statistical analysis was carried out by one-way ANOVA (ns not significant, *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). Cropped representative northern blots are shown under the graphs. Blots were hybridized with PhCRT1 antisense probe, and then re-hybridized with Ph18S rRNA probe. b Graphs show the relative CRT levels (mean of three replicates and standard deviation) normalized to the Petunia glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels. Statistical analysis was carried out by one-way ANOVA ANOVA (ns not significant, *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). Cropped representative western blots are shown under the graphs. Original northern and western blots are shown in the Fig. S1 (Supplementary information)

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