Table 2 Gene expression responses of marker genes to validate stress treatments. Transcriptional changes of selected stress marker genes in Arabidopsis, rice and barley. Values represent log2 fold-changes. Details for the references identifying these as markers and full gene descriptions can be seen in ST1

AOX1a

At3g22370

alternative oxidase 1A

4.85

4.71

1.93

1.78

1.92

3.93

AOX1c

At3g27620

alternative oxidase 1C

−0.1

−0.3

−2

−1.3

−1

0.3

Cys-2

AT5G09570

AT12CYS-2

2.66

4.72

0.22

0.78

−0

3.22

ABF3

AT4G34000

ABA responsive elements-BF3

0.32

0.79

4.04

1.61

0.42

1.38

RD29b

AT5G52300

RESPONSIVE TO DESICCATION 29B

0.34

0.85

9.15

3.73

−0.2

1.06

ERF1

AT3G23240

ethylene response factor 1

3.83

4.1

0.91

5.23

4.13

9.99

PDF1.2

AT5G44420

plant defensin 1.2

1.08

1.38

0

−2

−1.8

3.45

PR-1

AT2G14610

pathogenesis-related gene 1

0.44

1.38

0.53

0.82

7.56

−2

gene

AGI

Annotation

3AT (3 h)

AA (3 h)

ABA (3 h)

MV (3 h)

SA (3 h)

UV (3 h)

AOX 1A

LOC_Os04g51150

alternative oxidase 1A

6.05

3.28

1.2

3.12

3.01

8.46

AOX 1B

LOC_Os04g51160

alternative oxidase 1B

8.86

5.56

2.73

6.08

4.77

10.9

AOX 1C

LOC_Os02g47200

alternative oxidase 1C

−0.5

−0.5

1.17

0.05

0.05

−1.3

RAB16A

LOC_Os11g26790

Similar to RAB21

0.15

2.19

11.2

0.27

−1.2

1.62

NDB2

LOC_Os05g26660

NADH-ubiquinone oxidoreductase

1.4

1.84

1.25

1.25

1.97

2.82

NDA2

LOC_Os01g61410

NADH-ubiquinone oxidoreductase

10.6

3.55

−0.7

7.26

5.27

10.5

Putative orth.

LOC_Os03g51459

expressed protein (At2g21640 orth.)

4.44

1.89

2.37

0.46

3.44

3.95

gene

AGI

Annotation

3AT (3 h)

AA (6 h)

ABA (3 h)

MV (6 h)

SA (3 h)

UV (3 h)

AOX1A

HORVU2Hr1G101980

alternative oxidase 1A

6.61

4.79

2.24

0.1

2.19

4.66

Hv_AOX1C

HORVU6Hr1G068150

alternative oxidase 1C

0.2

−0.2

−0.7

−1.2

0.69

−1.3

AOX1D1

HORVU0Hr1G005420

alternative oxidase 1D1

3.84

2.37

−4.5

1.25

−2.7

11.4

AOX1D2

HORVU2Hr1G101990

alternative oxidase 1D2

2.87

1.24

−4.6

−2.6

−2.3

9.97

ABA resp.

HORVU5Hr1G077880

Beta-glucosidase 31

5.58

0.54

3.28

1.51

7.12

1.19

Hv_NDB2

HORVU3Hr1G076920

NAD(P)H-ubiquinone oxidoreductase B3

1.62

4

−2.7

1.94

−0.3

6.24

Hv_NDB3

HORVU7Hr1G073050

NAD(P)H-ubiquinone oxidoreductase B3

2.53

1.93

−1.1

−0.4

0.16

3.71

Stress resp. 3

HORVU5Hr1G087880

Auxin efflux carrier family protein

6.13

2.02

0.19

2.39

4.51

4.17

Stress resp. 4

HORVU0Hr1G019630

expressed protein

6.98

1.95

−0

1.06

4.3

7.3

Stress resp. 6

HORVU5Hr1G097140

PPR-containing protein

NA

NA

1.1

5.53

0.85

0.07