Fig. 3

Transcriptome responses of orthologous genes in Arabidopsis, rice and barley following six different treatments. (a) The number of DEGs in Arabidopsis (At), rice (Os) and barley (Hv) that were orthologous and showed conserved or opposite responses to different stress treatments are indicated for each species. Conserved responses were defined as genes that were orthologous with at least one other species, that were also up- or down-regulated in transcript abundance in response to the treatment. An opposite response was defined as genes that were orthologous in at least one species, that showed the opposite response in one or both of the other species. Both conserved and opposite responses exclude any orthogroups in which genes displayed both up- and -down-regulation within the same orthogroup. Orthogroups were defined using Orthofinder as outlined in the methods section. (b) Heatmap of log2-transformed fold-changes for all orthogroups [24] that contain differentially expressed genes (DEGs) showing opposite and conserved responses (up/down-regulated) in all three species in response to the six treatments. 3AT = 3-amino-1,2,4-triazole; AA = antimycin A; ABA = abscisic acid; MV = methyl viologen; SA = salicylic acid; UV = ultraviolet radiation