Fig. 4

Enhancer 1 is sufficient to activate AGL-mediated transcription. A series of promoter constructs were generated by cloning the enhancer 1, a trimerized enhancer 1, and a trimerized enhancer 1 without the four CArG sites (ΔCArG 1,2,3,4) in front of the CaMV 35S minimal promoter. Promoter activity in the presence of AGL40-AGL90 dimer was measured as the ratio of promoter-GUS activity over luciferase activity (control for transfection efficiency). All promoter activities were normalized as fold increase over the 35S minimal promoter activity in the presence of AGL40-AGL90 dimer. The locations of CArG sites are denoted by open squares. Averages and standard deviations were calculated from 8 to 9 biological replicates and two technical replicates for each biological sample