Fig. 2

InvINH1 promoter deletion assay. A series of 5′ promoter deletion constructs (D1 to D8) was generated by making 100-200 bp deletions from the 5′ end of the full-length InvINH1 promoter (FL). Promoter activity in the presence of AGL40-AGL90 dimer was measured as the ratio of promoter-GUS activity over luciferase activity (control for transfection efficiency). All promoter activities were normalized as the percentage of full-length promoter activity in the presence of AGL40-AGL90 dimer. The shaded boxes on the full-length promoter indicate the location of promoter region 1 (− 741 to -524 bp) and region 2 (− 453 to -265 bp). Averages and standard deviations were calculated from 2 to 8 biological replicates and two technical replicates for each biological sample