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Fig. 2 | BMC Plant Biology

Fig. 2

From: Thymol improves salinity tolerance of tobacco by increasing the sodium ion efflux and enhancing the content of nitric oxide and glutathione

Fig. 2

The effect of thymol on the accumulation of ROS in tobacco leaves and roots under salt stress. Tobacco seedlings were treated with water (C), 150 mM NaCl (S), 50 μM thymol (T) or the mixture of both (S + T) separately for 72 h. The roots were stained with DAB (a) or NBT (b) for 20 min to indicate H2O2 and \({\mathrm{O}}_2^{\bullet -}\) content, respectively. The seedlings were stained with DAB (c) or NBT (d) for 6 h to indicate H2O2 and \({\mathrm{O}}_2^{\bullet -}\) content in leaves. The activities of (e) SOD, (f) POD and (g) CAT. Different lowercase letters in e-g, indicated that the mean values of three replicates were significantly different between the treatments (P < 0.05, ANOVA, LSD)

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