Fig. 7

Over-representation of P. kurroa stress-responsive proteome compared with a customized reference of nine green plant species viz. Z. mays, G. max, A. thaliana, S. tuberosum, T. aestivum, N. tabacum, P. sativum, O. sativa and H. vulgare. A Number of P. kurroa stress-related proteins into diverse functional categories. Protein functions were predicted and categorized using AgriGO v. 2.0. B Validation of protein expression data of selected stress-responsive marker proteins in different P. kurroa organs using western blotting. Approximately, 25 µg of proteins from inflorescence, shoot, root and rhizome were separated by 12.5% SDS-PAGE, transferred to a PVDF membrane, and probed with antibodies against catalase, heat shock protein 70, type 1 chlorophyll a/b binding protein and 14-3-3 GRF. Actin was used as a loading control. The experiment was performed thrice with similar results. C Determination of picrosides content (PI-IV) in different organs at two developmental stages of P. kurroa. The abbreviations used are follows: IF, Inflorescence; ES, Early shoot; LS, Late shoot; ER, Early root; LR, Late root; ERh, Early rhizome; LRh, Late rhizome. Data are represented as the means ± S.D (n = 3) and different letters indicate statistically significant differences using one-way ANOVA followed by Tukey HSD post-hoc test (p < 0.05) for each variable autonomously in R v. 4.0.0