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Table 2 The predicted targets of highly induced tRFs by F. graminearum

From: tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight

tRF

Target_Acc.

Exp.

log2(CSI/CSM)

p value

log2(SMI/SMM)

p value

Annotation

tRFAla(CGC)

TraesCS6D02G244300

2.5

-0.45

0.5722

-0.25

0.2465

Dual specificity protein kinase YAK1

tRFAla(CGC)

TraesCS4B02G385200

2.5

-1.09

0.0553

-1.05

0.0370

Homeobox-leucine zipper protein

tRFAla(UGC)

TraesCS2A02G097900

2.5

-0.71

0.0304

-0.24

0.2946

DNA-directed primase/polymerase protein

tRFAla(UGC)

TraesCS2B02G113600

2.5

-0.80

0.0752

-0.92

0.0008

DNA-directed primase/polymerase protein

tRFCys(GCA)

TraesCS7B02G203400

2

-2.64

0.0024

-1.93

0.0025

ABC transporter B family member 19-like

tRFCys(GCA)

TraesCS7D02G298600

2

-2.26

0.0432

-1.25

0.0038

ABC transporter B family member 19-like

tRFCys(GCA)

TraesCS6B02G170700

2

-0.68

0.0001

-0.57

0.0133

ABC transporter B family member 19-like

tRFCys(GCA)

TraesCS6D02G131900

2

-1.76

0.0045

-1.07

0.0029

ABC transporter B family member 19-like

tRFGlu(CUC)

TraesCS6B02G032300

2.5

-1.34

0.0015

-0.68

0.1562

Pentatricopeptide repeat-containing protein

tRFGlu(CUC)

TraesCS6D02G026900

2.5

-0.82

0.0046

-0.69

0.0073

Pentatricopeptide repeat-containing protein

tRFGlu(CUC)

TraesCS3A02G085700

2.5

-0.75

0.0286

-0.35

0.3106

Trehalose 6-phosphate phosphatase

tRFGlu(CUC)

TraesCS3D02G085800

2.5

-0.77

0.0572

-0.95

0.0630

Trehalose 6-phosphate phosphatase

tRFGlu(CUC)

TraesCS6D02G395700

2.5

-0.93

0.1064

-0.48

0.1659

Interleukin-1 receptor-associated kinase 4

tRFGlu(CUC)

TraesCS5A02G002500

2.5

-2.40

0.0101

-0.64

0.2172

1-phosphatidylinositol-4-phosphate 5-kinase

tRFGlu(CUC)

TraesCS5D02G002700

2.5

-2.47

0.0567

-1.29

0.0146

1-phosphatidylinositol-4-phosphate 5-kinase

tRFHis(AUG)

TraesCS7B02G416600

2

-1.51

0.0241

-0.17

0.6981

Disease resistance protein RPM1

tRFHis(AUG)

TraesCS6A02G222000

2.5

-0.40

0.1980

-0.64

0.0465

Phytosulfokine receptor 1

tRFHis(AUG)

TraesCS6B02G291900

2.5

-1.37

0.0137

-0.97

0.0527

ABC transporter B family member 2-like

tRFiMet(CAU)

TraesCS2B02G598800

2.5

-0.88

0.0015

-0.79

0.0010

Polyadenylate-binding protein 4-like

tRFiMet(CAU)

TraesCS2B02G045400

2.5

-0.50

0.0429

-0.49

0.0229

Disease resistance RPP13-like protein 1

tRFiMet(CAU)

TraesCS2A02G030000

2.5

-1.18

0.0081

-0.91

0.0028

Disease resistance RPP13-like protein 1

tRFiMet(CAU)

TraesCS2B02G044900

2.5

-0.79

0.0047

-0.49

0.0058

Disease resistance RPP13-like protein 1

tRFiMet(CAU)

TraesCS2A02G029800

2.5

-0.43

0.0097

-0.34

0.0460

Disease resistance RPP13-like protein 1

tRFiMet(CAU)

TraesCS2D02G337300

2.5

-0.28

0.0164

-0.09

0.5289

Translation initiation factor 4B

tRFiMet(CAU)

TraesCS2A02G325400

2.5

-0.27

0.0781

-0.15

0.2779

Translation initiation factor 4B

tRFLeu(AAG)

TraesCS3B02G590000

2

-0.24

0.0213

-0.26

0.0180

UDP-D-apiose/UDP-D-xylose synthase

tRFLys(CUU)

TraesCS6A02G164200

2

-0.37

0.0065

-0.24

0.0506

Phosphatidylcholine transfer protein SFH2-like

tRFLys(CUU)

TraesCS1D02G264300

2.5

-0.71

0.0127

-0.45

0.0158

Ubinuclein

tRFLys(CUU)

TraesCS6D02G254900

2.5

-1.31

0.0004

-1.06

0.0001

Histone-lysine N-methyltransferase SUVR5

tRFLys(CUU)

TraesCS6A02G274600

2.5

-1.11

0.0007

-1.05

0.0001

Histone-lysine N-methyltransferase SUVR5

tRFLys(CUU)

TraesCS6B02G302100

2.5

-1.94

0.0010

-1.45

0.0006

Histone-lysine N-methyltransferase SUVR5

tRFLys(CUU)

TraesCS6D02G041400

2.5

-1.43

0.1912

-1.23

0.1284

Disease resistance protein RPS2

tRFLys(CUU)

TraesCS6B02G341700

2.5

-1.00

0.0183

-0.70

0.0056

TORTIFOLIA1-like protein 3

tRFLys(CUU)

TraesCS4D02G211200

2.5

-0.43

0.0111

-0.45

0.0164

Digalactosyldiacylglycerol synthase

tRFLys(CUU)

TraesCS4A02G093900

2.5

-0.71

0.0031

-0.53

0.0124

Digalactosyldiacylglycerol synthase

tRFLys(CUU)

TraesCS4B02G210400

2.5

-1.74

0.0005

-1.21

0.0002

Digalactosyldiacylglycerol synthase

tRFLys(CUU)

TraesCS1A02G341400

1

-1.04

0.2031

-0.31

0.5059

E3 ubiquitin-protein ligase

tRFLys(CUU)

TraesCS1D02G343700

2.5

-1.55

0.1171

-0.38

0.4265

E3 ubiquitin-protein ligase

tRFLys(CUU)

TraesCS1B02G354100

0

-1.33

0.0733

-0.94

0.0969

E3 ubiquitin-protein ligase

tRFLys(UUU)

TraesCS4B02G253500

2.5

-1.39

0.0016

-0.93

0.0001

Alpha-L-fucosidase 2

tRFLys(UUU)

TraesCS4B02G253300

2.5

-0.80

0.0027

-0.66

0.0004

Alpha-L-fucosidase 2

tRFLys(UUU)

TraesCS4D02G253400

2.5

-1.05

0.0008

-0.74

0.0028

Alpha-L-fucosidase 2

tRFLys(UUU)

TraesCS4A02G051000

2.5

-0.80

0.0068

-0.76

0.0018

Alpha-L-fucosidase 2

tRFPro(CGG)

TraesCS5A02G096600

0.5

-0.19

0.3818

-0.12

0.5391

Mitochondrial carrier protein

tRFPro(CGG)

TraesCS7B02G437300

2.5

-0.53

0.0923

-0.54

0.0429

Disease resistance protein RPM13

tRFThr(CGU)

TraesCS2B02G167000

1.5

-1.31

0.0001

-1.18

0.0008

Transcription-associated protein

tRFThr(CGU)

TraesCS2A02G141800

1.5

-1.40

0.0001

-1.18

0.0005

Transcription-associated protein

tRFThr(CGU)

TraesCS2D02G145300

1.5

-1.77

0.0001

-1.47

0.0012

Transcription-associated protein

tRFThr(CGU)

TraesCS3D02G364200

2.5

-0.94

0.0004

-0.74

0.0011

WD40 domain contain protein

tRFThr(CGU)

TraesCS3A02G371100

2.5

-0.78

0.1390

-0.62

0.5027

WD40 domain contain protein

tRFThr(CGU)

TraesCS1B02G075900

2.5

-0.87

0.2030

-0.33

0.6064

DNA repair protein RAD51 like protein

tRFThr(CGU)

TraesCS1D02G059600

2.5

-0.97

0.4057

-0.33

0.6472

DNA repair protein RAD51 like protein

tRFTrp(CCA)

TraesCS5D02G256900

2.5

-0.66

0.0037

-0.33

0.0131

tRNA (cytosine(34)-C(5))-methyltransferase

tRFTrp(CCA)

TraesCS5B02G247600

2.5

-0.82

0.0083

-0.43

0.0256

tRNA (cytosine(34)-C(5))-methyltransferase

tRFTyr(AUA)

TraesCS4A02G406400

0

-0.49

0.0106

-0.50

0.0318

Dynein assembly factor 1

tRFTyr(AUA)

TraesCS1B02G371300

2.5

-0.43

0.3945

-0.39

0.3175

Alpha-1,3-arabinosyltransferase XAT2-like

tRFVal(AAC)

TraesCS1D02G283000

1.5

-0.73

0.3259

-1.41

0.2318

Laccase

tRFVal(AAC)

TraesCS7B02G408700

2

-2.33

0.1853

-0.56

0.2495

ARR transcriptional factor

tRFVal(AAC)

TraesCS7B02G194500

2.5

-0.82

0.0113

-0.37

0.1357

Galactan beta-1,4-galactosyltransferase GALS1

tRFVal(AAC)

TraesCS4A02G444300

2.5

-0.89

0.0013

-0.75

0.0003

Phosphoglucan, water dikinase

  1. The targets of tRFs were analyzed using psRNATargets analysis server (http://plantgrn.noble.org/psRNATarget/analysis). The list of tRF sequences were submitted and the cDNA library of Triticum aestivum for target search.