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Fig. 7 | BMC Plant Biology

Fig. 7

From: tRNA-derived fragments from wheat are potentially involved in susceptibility to Fusarium head blight

Fig. 7

Experimental verification of predicted tRFs’ targets. (a) Relative expression levels of 12 target genes for the tRFLys, tRFThr, tRFGlu and 2 hub target genes. Expressional values are shown as means ± standard errors (s.e.) over three replicates. The TaActin genes was used as a loading control. Student’s t-test was used to compare the difference between F. graminearum (CSI or SMI) and mock (CSM or SMM) inoculations of the same variety. ***, P < 0.001; **, P < 0.01; *, P < 0.0 5. (b) 5’ RACE validation of the target genes. Vertical arrows indicate the cleavage site and the frequency of clones

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