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Fig. 1 | BMC Plant Biology

Fig. 1

From: Gene expression programs during callus development in tissue culture of two Eucalyptus species

Fig. 1

Callus induction, development, and transcriptome sequencing. (A) Experimental design of the callus induction and development. Stem tissues (A1, B1) were incubated on the CIM for dedifferentiation to get primary callus (A2, B2, pri-callus), which were further incubated on CIM for maturation (A3, B3, mat-callus). Mature calluses were transferred onto the SIM for 10 days incubation to expand the buds (A4, B4), as the shoot regeneration stage callus (SRS-callus). While mature calluses incubated on the CIM for long time (14 days) would lose the regeneration ability, which were called senescence callus (A5, B5, sen-callus). (B) Physiological experiments of callus during the incubation. Left and right panels are the weight and regeneration rates of the callus incubated on the medium for different time lengths. (C) Venn diagram of genes identified in the two Eucalyptus species. (D) Venn diagrams of genes identified in the callus tissues during the development of E. camaldulensis (left) and E. grandis x urophylla (right). (E) Correlation heat maps of the samples based on the gene expression profiles of callus tissues during the development of the two Eucalyptus species. (F) Combined correlation heat map of all the samples used in this study

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