Fig. 3

Varying frequencies of herbivory and CaLas inoculation modulate the expression of DMR6 and the accumulation SA hydroxylated molecules in mature leaves of C. sinensis. CaLas infection and feeding by D. citri induces changes in expression patterns of DMR6 (A-C), as well as, 2,3- and 2,5-DHBA accumulation in mature leaves of C. sinensis (D-I). Expression levels were normalized using two references genes, β-Actin and Elongation factor 1α. Data represent mean relative expression ± standard deviation (SD) of six biological replicates per treatment. White bars represent plants exposed to Non-infected (NI) D. citri, while black bars denote plants exposed to CaLas-infected (I) D. citri. Statistical analysis was performed with repeated measures ANOVA and Tukey post-hoc tests. Different letters indicate significant differences between the groups (P < 0.05)