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Fig. 1 | BMC Plant Biology

Fig. 1

From: Salicylic acid mediated immune response of Citrus sinensis to varying frequencies of herbivory and pathogen inoculation

Fig. 1

Varying frequencies of herbivory and CaLas inoculation modulate SA-related gene expression and SA accumulation in mature leaves of C. sinensis. CaLas infection and feeding by D. citri induces changes in expression patterns of NPR1, NPR3, NPR4, and PR-1, as well as, SA accumulation in mature leaves of C. sinensis. Expression patterns of genes involved in SA immune defense (A-L), SA accumulation in mature leaves (M-O). Expression levels were normalized using two references genes, β-Actin and Elongation factor 1α. Data represent mean relative expression ± standard deviation (SD) of six biological replicates per treatment. The gray dotted line denotes the level of SA accumulation at time zero in uninfected citrus plants. White bars represent plants exposed to Non-infected (NI) D. citri, while black bars denote plants exposed to CaLas-infected (I) D. citri. Statistical analysis was performed with repeated measures ANOVA and Tukey post-hoc tests. Different letters indicate significant differences between the groups (P < 0.05)

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