Fig. 4From: Spatiotemporal auxin distribution in Arabidopsis tissues is regulated by anabolic and catabolic reactions under long-term ammonium stressExpression of genes involved in auxin biosynthesis in the tissues of Arabidopsis thaliana plants grown on NO3− (control) or NH4+ as the only nitrogen source. Transcript levels of tryptophan aminotransferase of Arabidopsis (TAA1; A) and TAA-related 2 (TAR2; B). C Protein level of YUC1 (46 kDa) in leaves and roots. The western blot shown is representative of three independent replicates. Transcript levels of YUCCA family of flavin monooxygenases (YUC1-11; D, E) in leaves and roots. Transcript abundance values are normalized to the means of the reference gene PP2A. Relative expression was set to 1 in control plants for reference. Means are given with their SD (n = 6). Asterisks represent significant differences between NO3− versus NH4+-derived tissues (P < 0.05)Back to article page