Fig. 8

Effect of transient overexpression of the ScPIP2–1 gene in Nicotiana benthamiana leaves. a The chlorosis and DAB staining of N. benthamiana leaves after 24-h salt stress. b and c SPAD value and ion leakage in the N. benthamiana leaves after 24-h salt stress. SPAD represents the amount of chlorophyll, and ion leakage represents the value of conductivity. d Expression of three tobacco osmotic stress-related marker genes in the N. benthamiana leaves after 24-h salt stress. The NtEF1-α gene was used for data normalization. All data points are presented as means ± standard error (n = 3). Different lowercase letters indicate a significant difference, as determined with Duncan’s test (P < 0.05)