Fig. 10
Comparison between appearance and transections of the trinuclear stage anthers and pollen grains in Normal and HT-ms plants. a The morphology of Normal (above the white line) and HT-ms (below the white line) anthers at the trinuclear stage. Anther (b) and pollen grains (c) from a Normal plant stained with KI-I2 solution. Anther (d) and pollen grains (e) from a HT-ms plant stained with KI-I2 solution. f Cross sections of a Normal locule at the trinuclear stage of development. g Cross sections of a HT-ms locule at trinuclear stage of development. Cross sections were stained for 20 min with 1% SafraninO, followed by staining for 40s–60s with 1% Fast Green. Deh, dehiscence. Bars = 2 cm (a), 200 μm in (b and d), 100 μm in (c, e, f, g)