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Fig. 12 | BMC Plant Biology

Fig. 12

From: Functional characterization of hydroxyproline-O-galactosyltransferases for Arabidopsis arabinogalactan-protein synthesis

Fig. 12

Pollen phenotypes of the higher-order Hyp-GALT mutants. A and B Representative images of in vitro pollen assay showing pollen tubes from Hyp-GALT mutants in pollen germination medium after 3 h (column A) and pollen germination medium supplemented with 30 μM β-Gal Yariv after 1.5 h (column B). Scale bar = 100 μm. C and E represent the pollen germination percentages and pollen tube lengths of Hyp-GALT mutants in pollen germination medium. D and F represent the pollen germination percentages and pollen tube lengths of Hyp-GALT mutants in pollen germination medium supplemented with 30 μM β-Gal Yariv. For each measurement made in B, C, D, and E, twenty flowers of each genotype and 5 pollen tubes for each flower were measured using Image J. The experiment was repeated five times (total n = 500), and the values were subjected to statistical analysis by ANOVA, followed by the Tukey’s honestly significant difference test. (*, P < 0.05; **, P < 0.01; ***, P < 0.001). G The in vitro pollen germination assay showed defective pollen (indicated by arrows) in higher-order Hyp-GALT mutants compared to WT (A) and percent defective pollen in each genotype was calculated and quantified in (G)

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