Fig. 1

Single T-DNA insertional mutant information, the strategy for generating higher-order Hyp-GALT mutants and the list of mutants obtained after screening. A Schematic gene models for GALT2, GALT5, GALT7, GALT8 and GALT9, including the locations of T-DNA mutant insertions and primers used for PCR and qRT-PCR. The exon/intron structures are indicated; introns are drawn as lines and exons are drawn as rectangles, with blue rectangles representing coding sequences and orange rectangles representing UTRs. Sites of T-DNA insertions (marked as triangles) and locations of PCR primer sequences (grey arrowheads above the genes) and qRT-PCR primer sequences (red arrowheads below the genes) used for PCR screening and qRT-PCR, respectively, are indicated. The grey arrowheads above the T-DNA insertion indicate the location of the LBb1.3 T-DNA primer. The predicted GALECTIN (Pfam 00337) and GALT (Pfam 01762) domains are denoted by dashed lines. Information on Hyp-GALTs genes and their protein lengths ranging from 338 to 741 amino acids are provided in Supplementary Table 1. B The crossing strategy for generating a set of higher-order Hyp-GALT gene mutants. C Confirmation of the genotype of the galt2 galt5 galt7 galt8 galt9 quintuple mutant (marked as Q) by PCR. For example, galt2–2 genotyping produced a single band of 449–779 bp in size from the homozygous galt2 allele (*), whereas the WT allele (WT) produced a single band of 997 bp in size. Genotyping primer information and the sizes of the expected amplicon of each mutant allele are provided in Supplementary Table S2. D List of higher-order Hyp-GALT mutants and the key used in this study for all subsequent figures