Fig. 5
Expression of RWP-RK transcription factor UMSL057_0048 during gametogenesis. a RT-qPCR analysis of UMSL057_0048 expression levels compared to the control group (NCNW) in the first 48 h of gametogenesis. Three biological replicates were used for analysis and the gene expression level was calculated using the standard ddCt method. b Expression level of UMSL057_0048 at 6 h in induced (CW and NCW) versus not induced (CNW and NCNW) thalli. Three biological replicates were used for the experiment and the standard ddCt method was applied for analysis. Different lower case letters indicate significant differences among treatments (one-way ANOVA, P < 0.05). Error bars represent the standard error (n = 3)