Table 1 Expressed EST–STS and PLUG marker list W19513
Marker | Type | Primer (5′-3′) | Location | Geltype/Restrictionenzyme | Tm °C/t (h) |
|---|---|---|---|---|---|
TNAC1300 | PLUG | F: TCTGCAGGTTCGGTAGACAAT | 3AS 3BS 3DS | 2% agarose gel/TaqI | 60 |
R: AGTACGGGAGGACGCATGT | |||||
TNAC1341 | PLUG | F: GTTGAAGCCTACATGCCACAC | 3AL 3BL 3DL | 2% agarose gel/TaqI | 54 |
R: TAGCATGGGCTCCTAACATTG | |||||
TNAC1383 | PLUG | F: GCGGTCGATCTTCTTCAAGTC | 3AS 3BS 3DS | 2% agarose gel/TaqI/HaeIII | 60 |
R: TCAGATGGACTATGGGAGCAC | |||||
TNAC1627 | PLUG | F: CAGGAGGCCTACGAGACG | 3AS 3BS 3DS | 2% agarose gel/TaqI/HaeIII | 60 |
R: TTCTTCAGCTCGGATATTTGG | |||||
TNAC1364 | PLUG | F: CGTCAGGCTCAGGGTGTC | 3AL 3BL 3DL | 2% agarose gel/TaqI | 60 |
R: AAAGAGCCTCTGTCTCTCAGG | |||||
BM134465 | EST-SSR | F: CAATTGAACCCATCCGAAAG | 3AL 3BL 3DL | 8% non-denaturing | 60 |
R: CTGCCCGAACTATCCACAAT | polyacrylamide gel 8% non-denaturing/− | ||||
CD343475 | EST-SSR | F: CCGAAGACGAAGTCGAGAAC | 3AS 3BS 3DS | 8% non-denaturing | 62 |
R: ACACATCCCGTCCTTCTTTG | polyacrylamide gel 8% non-denaturing/− | ||||
BQ169491 | EST-SSR | F: TGGCCATCATTGAACTGAAA | 3AL 3BL 3DL | 8% non-denaturing | 56 |
R: CAATCAGATTTTTCGGCCAT | polyacrylamide gel 8% non-denaturing/− | ||||
CD452844 | EST-SSR | F: AAAAGTTGGCCACCAATCTG | 3AL 3BL 3DL | 8% non-denaturing | 60 |
R: TGGCATATGTCGCTCTGAAG | polyacrylamide gel 8% non-denaturing/− | ||||
BF429203 | EST-SSR | F: CTTCGTAGCCTCCTCACTGG | 3AL 3BL 3DL | 8% non-denaturing | 60 |
R: AGATTATGTGCGTGCTGTGC | polyacrylamide gel 8% non-denaturing/− |