Table 5 The DEGs were detected in the wheat roots with 3-MA added to the salt stress treatment (p-value < 0.001 and |log2FoldChange| > 7)

TraesCS3B02G483500

0.1

0.8544

−7.8

3.49E-10

+

2416

protein_coding

beta-glucosidase 5-like isoform X1 [Aegilops tauschii subsp. tauschii]

TraesCS2A02G527700

0.6

0.15539

−8.3

1.04E-11

+

1724

protein_coding

chalcone synthase [Oryza officinalis]

TraesCS7D02G469600

−1.3

0.05025

−7.2

9.22E-19

–

402

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS5A02G548600

1.6

#######

−7.3

#######

–

1649

protein_coding

predicted protein, partial [Hordeum vulgare subsp. vulgare]

TraesCS2D02G300100

1.4

0.00225

−9.9

1.10E-21

+

1272

protein_coding

loricrin-like [Aegilops tauschii subsp. tauschii]

TraesCS2A02G411600

−1.6

0.07519

−7.4

1.27E-08

–

381

protein_coding

protease inhibitor-like protein [Triticum aestivum]

TraesCS4D02G360000

1.5

0.002

−8.7

2.21E-12

+

426

protein_coding

pEARLI1-like lipid transfer protein 1 [Aegilops tauschii subsp. tauschii]

TraesCS2B02G126300

−1.4

0.14191

−8

3.46E-05

–

1254

protein_coding

peroxidase 70 [Oryza sativa Japonica Group]

TraesCS1D02G331800

−1.6

0.08427

−7.9

1.29E-05

–

351

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS1D02G331900

−1.2

0.18531

−9.9

4.33E-09

–

441

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS7B02G317800

−2.4

#######

−7.2

4.86E-18

+

384

protein_coding

lipid transfer protein EARLI 1-like [Aegilops tauschii subsp. tauschii]

TraesCS7A02G417700

−4.4

#######

−8.2

2.90E-10

+

384

protein_coding

14 kDa proline-rich protein DC2.15 [Triticum urartu]

TraesCS1B02G135800

−3.7

0.00452

8.44

NA

–

719

protein_coding

metallothioneine type1, partial [Hordeum vulgare subsp. vulgare]

TraesCS7B02G298400

−2.5

0.00327

−9.6

4.47E-08

+

1266

protein_coding

peroxidase 2-like [Aegilops tauschii subsp. tauschii]

TraesCS2A02G301500

−0.8

0.17673

−8.1

3.16E-10

+

1132

protein_coding

loricrin-like [Aegilops tauschii subsp. tauschii]

TraesCS1D02G213400

−1.2

0.18376

−9.6

2.94E-09

+

345

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS2B02G053200

6.4

#######

−9.2

1.78E-09

+

1132

protein_coding

xylanase inhibitor protein 1-like [Aegilops tauschii subsp. tauschii]

TraesCS1D02G285900

−1.7

0.00192

−7.2

5.92E-07

+

828

protein_coding

uncharacterized protein LOC109748351 [Aegilops tauschii subsp. tauschii]

TraesCS7B02G384900

−1.6

0.19774

−8.2

4.81E-05

–

399

protein_coding

predicted protein [Hordeum vulgare subsp. vulgare]

TraesCS3D02G538500

0.4

0.30155

−7.2

9.03E-09

–

1422

protein_coding

pectin acetylesterase 5-like isoform X1 [Aegilops tauschii subsp. tauschii]

TraesCS7D02G464600

−1

0.00271

−8

2.11E-18

–

1352

protein_coding

predicted protein [Hordeum vulgare subsp. vulgare]

TraesCS1D02G027300

−0.9

0.01898

−7.7

1.62E-19

+

300

protein_coding

Subtilisin-chymotrypsin inhibitor-2A [Triticum urartu]

TraesCS4D02G310500

0.3

0.76204

−7.8

5.71E-10

+

480

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS7A02G477100

−1.5

0.00078

−10

4.68E-15

–

1353

protein_coding

peroxidase 70-like [Aegilops tauschii subsp. tauschii]

TraesCS7A02G452900

0

0.9174

−7.3

2.93E-30

+

1299

protein_coding

peroxidase 39-like [Aegilops tauschii subsp. tauschii]

TraesCS7B02G384600

−3.6

0.00019

−8.2

3.60E-08

–

399

protein_coding

predicted protein [Hordeum vulgare subsp. vulgare]

TraesCS7A02G396400

−2

0.01087

−7.5

2.70E-10

+

1247

protein_coding

peroxidase 2-like [Aegilops tauschii subsp. tauschii]

TraesCS1D02G027400

1.4

0.00631

−7.6

3.71E−11

+

237

protein_coding

Subtilisin-chymotrypsin inhibitor-2A [Triticum urartu]

TraesCS3D02G467700

2.1

#######

-11

4.96E-14

+

1307

protein_coding

basic 7S globulin-like [Aegilops tauschii subsp. tauschii]

TraesCS7B02G273700

−1.7

0.02873

−7.6

2.35E-10

+

1563

protein_coding

berberine bridge enzyme-like 27 [Aegilops tauschii subsp. tauschii]

novel.8984

NA

NA

8.73

4.41E-12

+

401

–

–

TraesCS1B02G330200

−1.7

0.00063

−8.2

4.90E-10

–

1441

protein_coding

peroxidase 1-like [Aegilops tauschii subsp. tauschii]

TraesCS1B02G331200

−2.2

0.01578

−7.9

1.68E-08

+

1286

protein_coding

peroxidase 1-like [Aegilops tauschii subsp. tauschii]

TraesCS7B02G298600

−2.3

#######

−7.7

6.86E-09

+

1313

protein_coding

peroxidase 2-like [Aegilops tauschii subsp. tauschii]

TraesCS1D02G318000

−2.3

#######

−8.6

2.81E-11

+

1419

protein_coding

peroxidase 1-like [Aegilops tauschii subsp. tauschii]

TraesCS2A02G267200

−1.7

#######

−7.2

5.42E-11

–

548

protein_coding

hypothetical protein TRIUR3_15154 [Triticum urartu]

TraesCS1A02G329700

−0.6

0.59623

−9.7

2.03E-05

–

351

protein_coding

Cortical cell-delineating protein [Triticum urartu]

TraesCS2B02G482300

−0.9

0.56442

−7.2

0.0127

+

324

protein_coding

RecName: Full = Cold-regulated protein 2

TraesCS2D02G107800

8.4

#######

−9.7

6.98E-06

–

1256

protein_coding

peroxidase 1-like [Aegilops tauschii subsp. tauschii]

TraesCS7D02G469425

−1

0.0067

−9.4

1.03E-13

–

324

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCSU02G047800

1.1

#######

−10

1.30E-17

–

2025

protein_coding

unnamed protein product [Triticum aestivum]

TraesCS6B02G280400

−1.8

0.00011

−7.4

2.21E-08

+

2675

protein_coding

beta-D-xylosidase 3-like [Aegilops tauschii subsp. tauschii]

TraesCS2D02G108100

2.4

#######

−10

5.40E-11

–

1272

protein_coding

unnamed protein product [Triticum aestivum]

novel.13266

−2.3

#######

−8.3

4.98E-11

–

3879

–

uncharacterized protein LOC109771772 [Aegilops tauschii subsp. tauschii]

TraesCS2A02G040500

8.3

#######

−9.6

2.83E-13

–

906

protein_coding

xylanase inhibitor protein 1-like [Aegilops tauschii subsp. tauschii]

TraesCS1B02G330500

−2.2

#######

−8.3

9.69E-10

+

1425

protein_coding

peroxidase 1-like [Aegilops tauschii subsp. tauschii]

TraesCS3D02G318000

−2

#######

−8.3

2.41E-10

–

1296

protein_coding

blue copper protein-like [Aegilops tauschii subsp. tauschii]

TraesCSU02G144300

4.5

0.0004

−7.1

9.90E-05

+

390

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCSU02G144600

2.8

#######

−10

1.29E-15

+

465

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS4A02G412900

3.6

0.01668

−8.4

9.08E-05

+

426

protein_coding

Cortical cell-delineating protein [Triticum urartu]

TraesCSU02G144500

1.2

0.05018

−7.9

5.33E-11

+

432

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS7D02G049400

1.3

#######

−9.8

8.55E-16

–

1937

protein_coding

protein NRT1/ PTR FAMILY 2.3-like [Aegilops tauschii subsp. tauschii]

TraesCS1A02G356100

−1.5

0.00349

−7.5

8.00E-09

+

1194

protein_coding

peroxidase 1-like [Aegilops tauschii subsp. tauschii]

TraesCS4B02G368300

2.6

#######

−9.9

1.31E-14

–

432

protein_coding

predicted protein [Hordeum vulgare subsp. vulgare]

TraesCS7B02G298500

− 1.4

0.16193

−8.3

8.13E-09

+

1221

protein_coding

peroxidase 2-like [Aegilops tauschii subsp. tauschii]

TraesCS2D02G008100

−1.3

0.01092

−8.4

5.01E-10

+

1794

protein_coding

unnamed protein product [Triticum aestivum]

TraesCS2D02G108300

−1.4

0.00055

−7.4

9.36E-09

–

1291

protein_coding

unnamed protein product [Triticum aestivum]

TraesCS5A02G535500

3

#######

−9.4

9.50E-13

–

507

protein_coding

Cortical cell-delineating protein [Triticum urartu]

TraesCS7B02G010600

−1.9

#######

−8

2.78E-09

+

258

protein_coding

uncharacterized protein LOC109785281 [Aegilops tauschii subsp. tauschii]

TraesCS2D02G039300

7.7

#######

−7.1

5.07E-08

+

1201

protein_coding

xylanase inhibitor protein 1-like [Aegilops tauschii subsp. tauschii]

TraesCS1A02G318000

−2.9

#######

−7.1

3.60E-06

–

1300

protein_coding

peroxidase 1-like [Aegilops tauschii subsp. tauschii]

TraesCSU02G144800

5.8

#######

−9.4

4.04E-13

+

519

protein_coding

pEARLI1-like lipid transfer protein 2 [Aegilops tauschii subsp. tauschii]

TraesCS3A02G077100

NA

NA

7.41

4.33E-09

+

2245

protein_coding

asparagine synthetase [Triticum turgidum subsp. durum]

TraesCS1A02G318200

−2.8

#######

−7.1

1.20E-07

–

1241

protein_coding

peroxidase 1-like [Aegilops tauschii subsp. tauschii]

TraesCS4A02G409400

−1.5

0.01447

−7.1

5.76E-07

–

3492

protein_coding

MDIS1-interacting receptor like kinase 1-like [Aegilops tauschii subsp. tauschii]

TraesCSU02G243200

−2.7

#######

−7.1

8.95E-08

+

754

protein_coding

unnamed protein product [Triticum aestivum]

TraesCS7D02G539100

−1.7

0.00013

−8

3.00E-09

+

1344

protein_coding

tricetin 3′,4′,5′-O-trimethyltransferase-like [Aegilops tauschii subsp. tauschii]

TraesCS4A02G339000

1.6

#######

−9.3

3.20E-14

–

1876

protein_coding

4-hydroxyphenylacetaldehyde oxime monooxygenase-like [Aegilops tauschii subsp. tauschii]

TraesCS4B02G369000

4.5

0.00018

−9.7

1.14E-09

–

390

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS5A02G418600

−0.4

0.56518

−8.7

6.85E-06

+

609

protein_coding

predicted protein [Hordeum vulgare subsp. vulgare]

TraesCS2D02G458700

−4.4

0.1389

23.6

NA

–

651

protein_coding

hypothetical protein OsI_31610 [Oryza sativa Indica Group]

TraesCS1A02G209900

−0.4

0.36364

−8.7

1.23E-11

–

339

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS1D02G318600

−2.7

#######

−7.1

1.34E-06

+

1274

protein_coding

peroxidase 1-like [Aegilops tauschii subsp. tauschii]

TraesCS3A02G324700

−1.4

0.01242

−8

1.52E-08

–

894

protein_coding

unnamed protein product [Triticum aestivum]

TraesCS1B02G027800

0.2

0.68119

−7

2.04E-09

–

616

protein_coding

Bowman-Birk trypsin inhibitor-like protein [Triticum aestivum]

TraesCS7A02G054100

0.4

0.23441

−9

2.89E-13

–

1974

protein_coding

protein NRT1/ PTR FAMILY 2.3-like [Aegilops tauschii subsp. tauschii]

TraesCSU02G243695

4.8

0.00051

−7.7

2.25E-06

–

347

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

novel.2861

NA

NA

23.5

NA

+

1363

–

–

TraesCS2A02G002900

2.8

#######

−8.8

2.01E−12

–

1911

protein_coding

Cytochrome P450 86B1 [Triticum urartu]

TraesCS2A02G108000

-1

0.11668

−8.2

2.21E-08

–

1261

protein_coding

unnamed protein product [Triticum aestivum]

TraesCS4B02G313200

1.1

0.026

−8.1

1.13E-10

+

480

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS1A02G129600

4.2

0.00012

−7.1

3.97E-07

+

905

protein_coding

bidirectional sugar transporter SWEET3a [Aegilops tauschii subsp. tauschii]

novel.1280

−2.4

#######

−7.2

8.06E-07

–

2191

–

extensin-like [Aegilops tauschii subsp. tauschii]

TraesCS5D02G561700

0.7

0.1221

−7.9

5.31E-10

+

1302

protein_coding

aquaporin PIP2–2-like [Aegilops tauschii subsp. tauschii]

TraesCS7D02G410700

−1.7

0.0053

−7.5

2.99E-07

+

384

protein_coding

lipid transfer protein EARLI 1-like [Aegilops tauschii subsp. tauschii]

TraesCS6B02G018700

−0.3

0.43244

−8.3

6.09E-11

–

1220

protein_coding

extradiol ring-cleavage dioxygenase-like [Aegilops tauschii subsp. tauschii]

TraesCS2A02G108200

−1.5

#######

−7.6

6.68E-09

–

1328

protein_coding

unnamed protein product [Triticum aestivum]

TraesCS4D02G208900

4.3

#######

−7.1

9.72E-08

+

2126

protein_coding

laccase-10-like [Aegilops tauschii subsp. tauschii]

novel.13303

−1.8

0.0179

−7.3

6.61E-06

–

754

–

–

TraesCS6A02G137200

−2

0.00034

−7.1

6.16E-07

+

845

protein_coding

Early nodulin-like protein 2 [Triticum urartu]

TraesCS2B02G173200

−1.8

0.00115

−7.2

4.05E-07

+

1372

protein_coding

peroxidase 2-like [Aegilops tauschii subsp. tauschii]

TraesCSU02G242200

3.6

#######

−8

1.60E-09

+

390

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS1D02G021900

2.3

NA

−8.8

NA

–

378

protein_coding

Bowman-Birk trypsin inhibitor-like protein [Triticum aestivum]

TraesCS1D02G050600

2.3

0.01591

−8.8

6.75E-05

+

709

protein_coding

Salt stress-induced protein [Dichanthelium oligosanthes]

TraesCS3A02G460900

−1.9

0.00107

−7

1.21E−06

–

1708

protein_coding

probable leucine-rich repeat receptor-like protein kinase At5g49770 [Aegilops tauschii subsp. tauschii]

TraesCS7B02G002000

-0

0.94185

−8.1

2.61E-10

–

855

protein_coding

aquaporin PIP2–2-like [Aegilops tauschii subsp. tauschii]

novel.890

NA

NA

22.9

NA

+

1511

–

hypothetical protein TRIUR3_19550 [Triticum urartu]

TraesCS1D02G214500

4

0.04098

−8.8

0.00081

+

672

protein_coding

predicted protein [Hordeum vulgare subsp. vulgare]

TraesCS4D02G104000

5.2

#######

−8.8

3.83E-12

+

528

protein_coding

dirigent protein 21-like [Aegilops tauschii subsp. tauschii]

TraesCS5D02G004100

0

0.98706

−7.1

4.42E-08

+

447

protein_coding

puroindoline a [Triticum turgidum subsp. durum x Aegilops tauschii]

TraesCS7D02G020100

6

0.00022

−7.4

0.00281

–

1173

protein_coding

extensin-like isoform X1 [Aegilops tauschii subsp. tauschii]

TraesCS1D02G006600

3.6

#######

−8.7

2.09E-11

+

990

protein_coding

hypothetical protein TRIUR3_28534 [Triticum urartu]

TraesCS7B02G115300

4.8

#######

−7

9.21E-08

–

674

protein_coding

GASR7 [Triticum aestivum]

TraesCS2D02G152700

−0.2

0.77316

−7.8

9.40E-09

–

1540

protein_coding

peroxidase 2-like [Aegilops tauschii subsp. tauschii]

TraesCS5B02G528700

−0.4

0.47088

−7.6

3.86E-08

+

1187

protein_coding

expansin-A31-like [Aegilops tauschii subsp. tauschii]

TraesCS5A02G558000

−0.4

0.33336

−7.6

6.13E-09

–

1616

protein_coding

probable receptor-like protein kinase At4g10390 [Aegilops tauschii subsp. tauschii]

TraesCSU02G154100

5.5

0.00709

−8.6

0.0002

+

390

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS4B02G367800

1.8

0.07225

−8.2

1.50E-08

–

432

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

novel.3884

−0.8

0.06377

−7.3

7.32E-08

+

576

–

predicted protein [Hordeum vulgare subsp. vulgare]

TraesCS7D02G139500

0.9

0.17508

−7.7

1.47E-08

+

1554

protein_coding

uncharacterized protein LOC109732600 [Aegilops tauschii subsp. tauschii]

TraesCS3B02G405900

−0.2

0.79462

−7.1

1.10E-06

+

1153

protein_coding

unnamed protein product [Triticum aestivum]

novel.4897

2.4

0.37495

−7.7

0.04761

+

1614

–

predicted protein [Hordeum vulgare subsp. vulgare]

TraesCS2A02G002800

4.4

#######

−7.7

3.85E-09

+

1978

protein_coding

Cytochrome P450 86B1 [Triticum urartu]

TraesCS5D02G249900

1.3

0.1679

−7.4

2.04E-06

+

300

protein_coding

hypothetical protein TRIUR3_16342 [Triticum urartu]

TraesCS3D02G338600

0.9

0.16034

−7.3

2.80E-07

+

1292

protein_coding

expansin-A2 [Oryza sativa Japonica Group]

TraesCS5A02G243400

1.4

0.05229

−7.4

6.90E-08

+

288

protein_coding

hypothetical protein TRIUR3_16342 [Triticum urartu]

TraesCS1D02G050800

3.9

0.00035

−7.6

7.24E-08

+

792

protein_coding

LOW QUALITY PROTEIN: mannose/glucose-specific lectin [Setaria italica]

TraesCS1D02G050700

4

0.00047

−7.5

2.65E-07

+

486

protein_coding

LOW QUALITY PROTEIN: mannose/glucose-specific lectin [Setaria italica]

TraesCS7A02G452400

1.4

0.05297

−7.2

1.23E-07

+

1652

protein_coding

probable glycosyltransferase 4 [Aegilops tauschii subsp. tauschii]

TraesCSU02G185500

6.1

#######

−7.4

1.04E-07

+

390

protein_coding

cortical cell-delineating protein-like [Aegilops tauschii subsp. tauschii]

TraesCS5A02G270300

7

#######

−7.4

6.05E-08

+

2445

protein_coding

G-type lectin S-receptor-like serine/threonine-protein kinase [Panicum miliaceum]