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Table 3 Sequence characteristics of the four sequenced regions

From: Establishing community-wide DNA barcode references for conserving mangrove forests in China

 

rbcL

ITS2

matK

trnH-psbA

Total number of sequencesa

885

789

629

654

Success rate of PCR and sequencing

98.6%

87.9%

70.0%

72.8%

Number of speciesb

72

65

67

63

Percent with valid conspecifics

89.0%

90.8%

80.6%

88.9%

GC content

44.1

59.7

33.9

27.6

Number of variable sites

229

448

713

1072

Number of alignment gaps

NA

551

345

1402

Alignment length

570

679

902

1411

Maximum intraspecific distance

0.005

0.046

0.018

0.077

Maximum interspecific distance

0.267

0.705

0.835

1.481

  1. aAll sequences that have been successfully sequenced
  2. bSequences except for hybrids and variations