Skip to main content
Fig. 6 | BMC Plant Biology

Fig. 6

From: Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat

Fig. 6

Mannitol stress recovery (MAN-R8) relates to continuous MT regeneration and reorganization in embryogenic microspores. A1-A6 Uni-nucleate star-like structure (SLS). A1 Fine CMT, excluding in the vicinity of the nucleus where radial CMT assembled into thicker bundles. A2 Long strands of EMT in the cytoplasm, transecting the vacuole to the plasma membrane. A3 EMT strung together to form long and thick strands in the vicinity of the nucleus (N). A4-A6 3D model of the subcellular volume of a similar structure. Superior visualisation axis model (A4). Long strands of EMT encircled the nucleus (N). Volumetric ortho-slicer view from the forward (A5) and opposite site of the microspore model (A6). B1-B3 Bi-nucleate embryogenic structure. B1 Fine and short CMT without preferential orientation. B2 EMT assembled into long and thick bundles in the vicinity of the sister nuclei. B3 The radial EMT network extends out to the cell cortex. Cortex – CMT; z-series projected as a maximum intensity projection from cortex optical sections collected from the front (F) or from the back (B) side of microspore. Middle – optical section at the middle of the z-series. Middle+Cortex B – Endoplasmic and cortical MTs; z-series projected as a maximum intensity projection from middle and cortical optical sections collected from the back side (B) of microspore. Inserts on the upper-right corner in the first panels show a corresponding image in the differential interference contrast (DIC). Prj – z-series projected as a maximum intensity from the front side (Prj F) or from the back side (Prj B) of microspore. Green fluorescence (Alexa 488) shows α-tubulin. Red fluorescence of nuclei is caused by PI staining. Red autofluorescence of exine. Scales =20 μm

Back to article page