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Fig. 1 | BMC Plant Biology

Fig. 1

From: Epigenetic modifications potentially controlling the allelic expression of imprinted genes in sunflower endosperm

Fig. 1

Identification of imprinted genes in sunflower endosperm at 12 DAP. A and B Parental expression ratios plot for each reciprocal cross in SY1/YS1 (A) and SY2/YS2 (B). The expression levels of paternal (y-axis) and maternal (x-axis) alleles are represented by the log2 read counts of the paternally- and maternally-derived reads in the reciprocal crosses, respectively. The color scale in blue (low) and red (high) represents the relative density of the genes. The solid diagonal line represents the expected 2 m:1p ratio. C and D Venn diagram analysis of imprinted genes. The number of imprinted genes identified in two crosses are shown in the red (SY1/YS1) and blue (SY2/YS2) circles, respectively. E and F Comparison of imprinted genes in two crosses of sunflower. Non-imprinted: genes not showing significant deviation from 2:1 ratio of maternal allele to paternal allele in each reciprocal hybrid (Chi-square (2:1, q > 0.05)). Non-analyzed: genes without sufficient read counts. Low-stringency maternal transcripts (MEG and MNC)/paternal transcripts (PEG and PNC): transcripts showing significant deviation from 2:1 ratio of maternal allele to paternal allele in each reciprocal hybrid (Chi-square (2:1, q < 0.05)). High-stringency MEGs/PEGs: genes in which favorable alleles were at least five times more than those of non-favorable alleles in both directions of a reciprocal cross (q < 0.05 (χ2 test))

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