Fig. 6

Leaf traits of WT and catalytically inactive OsHXK1 transgenic lines. a Comparison of the leaf phenotypes of WT and catalytically inactive OsHXK1 transgenic lines (OsHXK1–D1 and OsHXK1–D3). Bars = 5 cm. b Detached leaves of WT, OsHXK1–D1, and OsHXK1–D3 plants at the flowering stage were incubated with water before and after 5 days of dark treatment. The data were obtained from three independent replicates. c OsHXK1 expression in WT and catalytically inactive OsHXK1 transgenic lines. Gene expression levels were measured relative to the rice OsActin1 transcript level. d Chlorophyll contents of detached leaves of WT and catalytically inactive OsHXK1 transgenic plants before and after dark treatment. The values are the means ± SDs of 10 measurements. *, 0.01 < P < 0.05, **, P < 0.01. The P value was determined by Student’s t-test. The error bars indicate SDs