Fig. 3

OsHXK1 participates in dark-induced leaf senescence. a-d OsHXK1 promoted dark-induced leaf senescence. Detached flag leaves from WT, OEHXK1–1, OEHXK1–2, Cashxk1–1, and Cashxk1–2 plants at the flowering stage were incubated with water, 50 μM ABA, and 6% glucose for 5 days in the dark. e Chlorophyll content in detached leaves of WT, OEHXK1–1, OEHXK1–2, Cashxk1–1, and Cashxk1–2 plants after different dark treatments. The values are the means ± SDs of 10 measurements. *, 0.01 < P < 0.05. **, P < 0.01. P values were determined by Student’s t-test. The error bars indicate SDs. f-h, Expression of some SAGs (OsSGR, Osh36, and Osl2) in detached leaves of WT and OsHXK1 mutant plants after different treatments. *, 0.01 < P < 0.05. **, P < 0.01. The P value was determined by Student’s t-test. The error bars indicate SDs