Fig. 2

Phenotypic characterization analysis of WT, OEHXK1–1, OEHXK1–2, Cashxk1–1, and Cashxk1–2. a Plants of the WT, OEHXK1–1, and Cashxk1–1 lines after the flowering stage. b Comparison of the flag leaf phenotype of WT, OEHXK1–1, OEHXK1–2, Cashxk1–1, and Cashxk1–2 plants. c-d, Chlorophyll fluorescence imaging and Fv/Fm ratio analysis of wild-type, OEHXK1–1, OEHXK1–2, Cashxk1–1, and Cashxk1–2 plants after the flowering stage. e TEM analysis of leaves of WT, OsHXK1-overexpressing, and CRISPR/Cas9 plants at the filling stage. GL, grana lamellae, SL, stroma lamellae, St, starch grain. f Chlorophyll content analysis of WT, OsHXK1-overexpressing, and CRISPR/Cas9 plant leaves from the booting to the mature stage. *, 0.01 < P < 0.05. **, P < 0.01. The error bars indicate SDs. Bars = 20 cm in a, 5 cm in b, and 1 μm in e