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Fig. 5 | BMC Plant Biology

Fig. 5

From: Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

Fig. 5

Southern hybridization of Arabidopsis T1 lines harboring the pMDC164 and pMDC43 constructs and moso bamboo wild-type plants. Genomic DNA isolated from untransformed/empty vector control (UC) plants did not show any hybridization signal. Stressed plants and non-stressed control (CK) plants, digested with Pac1 or HindIII enzyme showing hybridization signals, (A pMDC164, C pMDC43) PHRE1 and (B pMDC164, D pMDC43) PHRE2 5’ LTR sequences were used as probes for hybridization of specific blots. Different lane numbers represent different transformant plants. Arrowheads indicate the transposed copies of LTRs. E and F, Southern hybridization for moso bamboo wild-type plants under heat stress. Genomic DNA isolated from stressed plants and non-stressed control (CK) plants, digested with HindIII enzyme showing hybridization signals. (E) PHRE1 and (F) PHRE2 LTRs were used as probes for the hybridization of specific blots. Different lanes represent different plants

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