Fig. 4

In situ hybridization assay shows the expression patterns of PHRE1 and PHER2 in moso bamboo roots and leaves. Location of PHRE1 expression signified by purple/blue color due to the enzymatic cleavage of a chromogenic substrate (5- Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) by alkaline phosphatase-conjugated to anti-DIG antibody. (A, C-i-iii) In situ hybridization of cross-sections of primary and lateral roots, roots, and (B, D-i-iii) mature leaf blades with PHRE1 and PHRE2 antisense probes. (A, B, C, D-iv) negative control with sense probes. Roots and leaves were sampled from 30-d-old seedlings treated under heat stress at 45 ^oC for 4h. C, cortex; Ep, epidermis; P, pericycle; X, xylem; XP, xylem parenchyma; En, Endodermis and Gc, Guard cells. Scale bar represents 50 μm