Fig. 3

Transgenic Arabidopsis plants showing GUS expression analysis of PHRE1 and PHRE2. A, C GUS expression analysis of pMDC164 construct of PHRE1 and PHRE2 in transgenic Arabidopsis plants. Four weeks old T1 seedlings were incubated at 37 °C for 24h. (i) T1 transformants harboring pMDC164 construct with PHRE1 and PHRE2 (ii) transgenic plant under normal conditions and UC. B, D (i) GUS histochemical staining of T1 transformants harboring pMDC43 construct with PHRE1 and PHRE2. Four weeks old T1 seedlings were incubated at 37 °C for 24h, (ii) transgenic plants without stress, and UC. The blue color indicates the level of expression and the pMDC164 construct with PHRE1 and PHRE2 showed stronger expression than the pMDC43 construct with PHRE1 and PHRE2. UC, untransformed control plant. Scale bar represents 20 μm