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Fig. 5 | BMC Plant Biology

Fig. 5

From: Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata

Fig. 5

The relative transcript level of PtrINV genes in different tissue types of P. trifoliata during osmotic stresses. A-C The transcript profile of PtrINV genes in response to salt stress in leaves (A), stems (B) and roots (C) of two-month old P. trifoliata seedlings that exposed to 200 mM NaCl for indicated time (0 h, 6 h, 12 h, 24 h, 48 h and 72 h) and recovered for a day. (D-F) The transcript profile of PtrINV genes in response to dehydration stress in leaves (D), stems (E) and roots (F) of two-month old P. trifoliata seedlings that exposed to dehydration stress for indicated time (0 h, 0.5 h, 1 h, 2 h, 3 h and 5 h) and recovered a day. Real-time quantitative PCR (qRT-PCR) assay was performed using specific primers and PtrActin gene was utilized as a reference gene. The gene expression analysis for each time point was repeated for at least two times with four biological replicates, and representative data are shown. The relative gene expression was calculated using the 2-∆∆CT method relative to the initial treatment time (0 h) that set as 1. The normalized values of the expression levels were converted into Log2 of expression values and visualized by heatmap figure. Heatmap figures were constructed by TBtools based on default features, Euclidean distance method, and complete cluster method (n = 3)

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