Fig. 4

Tissue-specific expression profile of PtrINV genes in P. trifoliata. RNA was extracted from two-month old leaves (L), stems (S) and roots (R) of P. trifoliata seedlings, P. trifoliata flowers (W) of spring blossom, and matured P. trifoliata fruits (F). Real-time quantitative PCR (qRT-PCR) assay was performed using specific primers and PtrActin gene was utilized as reference gene. The transcript abundance was obtained based on standard curve using QuantStudio™ 7 Real-Time PCR Software v1.1 and normalization by the expression of PtrActin. Heatmap figure was constructed using Log₂ of gene expression data by TBtools based on default features: euclidean distance method, and complete cluster method (n = 3). The red color indicates to highly expression levels, and blue color shows low expression levels. The cluster dendrogram is shown on left side of the heatmap