Fig. 6

LcERF056 binds directly to GCC-box and DRE motifs in promoters to activate the transcription of downstream target genes. (A) Reporter and effector vectors used for yeast one-hybrid (YIH) experiments. (B) Verification of the interactions between LcERF056 and GCC-box and DRE cis elements through YIH experiments. Positive transformants were identified by spotting serial dilutions of yeast (1:1, 1:10, and 1:100) onto SD/−L-U plates supplemented with 400 ng/mL AbA. (C) Schematic structure of LcLTP, LcRP, and LcPrx genes. ATG, translation start point; Red line, GCC-box or DRE-box; Grey boxes, ChIP-qPCR amplified fragments that correspond to the regions indicated in the gene structure. (D) ChIP-qPCR analysis of the enrichment levels of LcLTP, LcRP, and LcPrx in LcERF056-OE and LcERF056-RNAi transgenic plants by using the specific synthetic antibody (rabbit). WT assay was included as control. *P < 0.05 between WT and transgenic plants, using Student’s t test