Inoculation methods | Inoculation sites | Volume of agroinfiltration liquid that can enter plants (μl) | Time required to obtain VIGS plants (d) | Success rate of VIGS (%) | Time required to obtain symptomatic plants (d) | Inoculation success rate of TYLCV (%) |
---|---|---|---|---|---|---|
INABS | no-apical-bud stem section | 100–200 | 10–12 | 60 | 8–10 | 72 |
Infiltration the dorsal leaf | back of tender leaf | 10–50 | 30–50 | 30 | 23–45 | 40 |
Injection of the basal stem | base of stem (~ 2–3 cm from the soil surface) | 20–30 | 35–60 | 36 | 30–45 | 48 |