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Table 1 Effects of bacterial concentration and inoculation time on the efficiency of INABS infiltration

From: A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings

Inoculation time

VIGS efficiency of the PDS gene (%) and TYLCV inoculation success rate (%) after infiltration of Agrobacterium at different concentrations

0.5 (OD600)

1.0 (OD600)

1.5 (OD600)

4 dpi

8 dpi

12 dpi

11.3b/16.7b

17.7c/27.7b

16.0b/23.3b

21.7a/34.3a

56.7a/68.3a

30.7a/56.0a

17.7b/29.3a

45.0b/61.0a

28.3a/55.0a

  1. Note: Each value represents the mean transformation efficiency or success rate of virus infection (n = 3). Within a column, significant differences (P ≤ 0.05) among treatments are indicated by different letters (Duncan’s multiple range test)