Fig. 2From: A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttingsQuantitative qRT-PCR analysis of TRV-mediated VIGS of the PDS gene in tomato leaves by injection of the no-apical-bud stem section (INABS). WT: wild-type plants; TRV: VIGS control plants infected by Agrobacterium tumefaciens carrying pTRV 2 and pTRV1; TRV-SlPDS: PDS-silenced plants infected by A. tumefaciens carrying pTRV2-SlPDS and pTRV1. Values (means ± SD are the averages of three independent experiments, and significant differences (P ≤ 0.05) among treatments in each group are indicated by different letters (Duncan’s multiple range test)Back to article page