Fig. 8

Allene Oxide Cyclase (AOC) silencing induced by TRV California based VIGS vectors in N. attenuata. A Plants inoculated with TRV California based VIGS vectors (pTRV-RNA1/pTRV-RNA2:AOC) for AOC silencing. Pictures were taken at 13 dpi (days post inoculation). B Relative AOC transcript abundance in the same plants compared to empty vector controls. Growth temperatures after inoculation (28 °C and 30 °C) are indicated. Apical leave samples for AOC transcript quantification were taken at 13 dpi and 5 h after treatment of the plants with Manduca sexta oral secretion (OS). Transcript abundance is shown as mean values ±SD (Student’s t-test, * P < 0.05)