Fig. 7

Phytoene desaturase (PDS) silencing efficiency in N. attenuata at different growth temperatures. A Bleaching phenotype of plants inoculated with TRV California (pTRV-RNA1/pTRV-RNA2:PDS; California) and PpK20 (pBINTRA6/pTVPD; PpK20) based VIGS vectors for PDS silencing. B Relative PDS transcript abundance in the same plants compared to empty vector controls (EV). Growth temperatures after inoculation (22 °C, 28 °C and 30 °C) are indicated. Pictures and samples for PDS transcript quantification were taken at 13 dpi (days post inoculation). C Relative PDS transcript abundance of plants inoculated with sap from N. attenuata pre-inoculated with the same plasmid combinations as above. Growth temperatures after sap-inoculation (28 °C and 30 °C) are indicated. Samples for PDS transcript quantification were taken from apical leaves at 20 dpi. Transcript abundance in B) and C) is shown as mean values ±SD (Student’s t-test, * P < 0.05 and ** P < 0.01)